semi dry transfer system (Bio-Rad)

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Bio-Rad semi dry transfer system
Semi Dry Transfer System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 468 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nitrocellulose+transfer+kit/Trans-Blot+Turbo+RTA+Mini+Nitrocellulose+Transfer+Kit/pmc13179526-79-10-13
Average 96 stars, based on 468 article reviews

semi dry transfer system - by Bioz Stars, 2026-09

96/100 stars

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Article Title: Reversible in vivo regulation of drug metabolizing enzyme CYP1A2 activity through a dTAG knock-in strategy
Article Snippet: Mini PROTEAN TGX Gels (4-20%), 10x Tris/Glycine/SDS buffer, Precision plus proteinTM all blue standards, and trans-blot turbo RTA mini 0.2 μm nitrocellulose transfer kit was obtained from Bio Rad (Hercules, CA).

Article Title: VPS13A Deficiency Leads to Impaired Lipid Distribution and Alteration of Mitochondrial Calcium Homeostasis in Fibroblasts of VPS13A Disease Patients.
Article Snippet: Blotting was performed using the Trans-Blot Turbo RTA Midi 0.2 μm Nitrocellulose Transfer Kit (Bio-Rad: 1704271).

Article Title: Aging alters DNA structure-induced genetic instability in mice.
Article Snippet: Proteins were transferred using the Trans- Blot Turbo Transfer System (Bio Rad, REF: 1704150) and the Trans- Blot Turbo RTA Midi 0.2 μm Nitrocellulose Transfer Kit (Bio Rad, REF: 1704271).

Staining:

Article Title: Parkinson’s disease linked LRRK2 G2019S drives oxidative nuclear DNA damage and PARP1 hyperactive signaling
Article Snippet: For western blots, whole cell brain lysates were incubated at 70°C for 5 minutes with NuPAGE sample loading dye (ThermoFisher Scientific, NP0007) and dithiothreitol (DTT) (Bio-Rad, 1610610). .. Protein lysates were run on 4-20% mini-protean TGX stain-free gels (Bio-Rad, 4568096) and transferred using the Trans-Blot Turbo Transfer System (Bio-Rad, 1704155) and Trans-Blot Turbo RTA Midi 0.2μm Nitrocellulose Transfer Kit (Bio-Rad, 1704271). .. Membranes were blocked in 5% w/v nonfat dry milk in PBST (PBS with 0.05% Tween 20) and incubated with primary antibodies/binding reagents diluted in 5% w/v nonfat dry milk in PBST at 4°C overnight (anti-poly-ADP-ribose binding reagent, Millipore Sigma, MABE1031, 1:10,000; Novus Biologicals, NBP1-47423, 1:10,000).

Article Title: Parkinson’s disease linked LRRK2 G2019S drives oxidative nuclear DNA damage and PARP1 hyperactive signaling
Article Snippet: For western blots, cell lysates were incubated at 100°C for 5 minutes with NuPAGE sample loading dye (ThermoFisher Scientific, NP0007) and dithiothreitol (Bio-Rad 1610610). .. Protein lysates were run on 4-20% mini-protean TGX stain-free gels (Bio-Rad, 4568096) and transferred using the Trans-Blot Turbo Transfer System (Bio-Rad 1704155) and Trans-Blot Turbo RTA Midi 0.2μm Nitrocellulose Transfer Kit (Bio-Rad, 1704271). .. Membranes were blocked in 5% w/v nonfat dry milk in PBST (PBS with 0.05% Tween 20) and incubated with primary antibodies/binding reagents diluted in 5% w/v nonfat dry milk in PBST at 4°C overnight (anti-poly-ADP-ribose binding reagent, Millipore Sigma, MABE1031, 1:10,000; anti-PARP1, Santa Cruz Biotechnology, sc-8007, 1:1,000; anti-XRCC1, Novus Biological, NBP1-87154, 1:5,000; anti-Pol β, Abcam, ab26343, 1:1,000; anti-Ligase III, GeneTex, GTX70143, 1:1,000; anti-H3, Abcam, ab1791, 1:20,000; anti-ɑ-tubulin, Abcam, ab7291, 1:10,000; anti-β-actin, Novus Biologicals, NBP1-47423, 1:10,000; anti-β-actin, Abcam, ab8227, 1:10,000).

Semi-Dry Trans-Blot:

Article Title: The Interaction with Nanotopographical Environment regulates nuclear mechanoresponse in mESCs via Histone Demethylase KDM3A
Article Snippet: PageRuler Plus Prestained Protein Ladder (Thermo Scientific, # 26619), was used as molecular weight marker. .. Proteins were transferred using the Trans-Blot Turbo RTA Mini 0.2 μm Nitrocellulose Transfer Kit (BioRad, #1704270) and the semi-dry Trans-Blot Turbo System (Bio-Rad, #1704150), for 30 min with the standard protocol. .. Transfer efficiency was verified by Ponceau S staining (0.1% Ponceau S (Roth, # 59382), 1% acetic acid (TH.

Activation Assay:

Article Title: Altered cellular VEGF dynamics in Chronic Obstructive Pulmonary Disease
Article Snippet: .. Following the gel activation, proteins were transferred on the membranes of the Trans-Blot Turbo RTA Mini 0.2 μm Nitrocellulose Transfer Kit (#1704270, Bio-Rad) using Trans-Blot® TurboTM Transfer System (#1704150, Bio-Rad) 1.3A, 200V for 10 min. ..

Membrane:

Article Title: Improve the Efficacy of B7-H3-Targeting Antibody-Drug Conjugate DS-7300a in TP53-deficient Tumors by Inducing Ferroptosis
Article Snippet: .. The cell pellets were then lysed in 1x Laemmli sample buffer (Bio-Rad Laboratories, 1610747), premixed with 2-mercaptoethanol (Bio-Rad Laboratories, 1610710), at 99 °C for 20 min. Proteins were separated with 4%−15% Mini-PROTEAN TGX Precast Protein Gels (BioRad, 4561086) and transferred to the nitrocellulose membrane using the Trans-Blot Turbo RTA Mini 0.2-μm nitrocellulose transfer kit (Bio-Rad Laboratories, 1704270). ..

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